Journal: Scientific Reports

Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways

doi: 10.1038/s41598-018-36374-4

Figure Lengend Snippet: Targeted GFP 1–10 sensors reveal subcellular pools of Tau. ( a ) The subcellular distribution of the indicated GFP 1–10 sensors in transiently transfected mouse C17.2 cells is shown by confocal microscopy upon immune staining of PFA-fixed cells with an anti-GFP antibody (upper row, in red). The cells are counter-stained with the ER-marker calnexin (middle row, in cyan) and the nuclear stain DAPI (shown in the merged images, bottom row, in blue). ( b ) The GFP 1–10 sensors were then co-transfected with S 11 -Tau, which reveals by biFC biofluorescence (upper row, in green) microtubule-associated Tau with GFP 1–10 , nuclear Tau with nucGFP 1–10 , and mitochondria-associated Tau with ommGFP 1–10 . No biFC is obtained with the erGFP 1–10 sensor, unless Tau is targeted to the ER lumen (erTau-S 11 , column on the far right). The cells are counter-stained for total human Tau with the Tau13 antibody (middle row, in red) and DAPI (merged images, bottom row, in blue).

Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), pATM (Cell Signaling, S1981, diluted 1:500), pChk1 (Cell Signaling, S345, diluted 1:500), pChk2 (Cell Signaling, T68, diluted 1:500), pATR (Cell Signaling, S428, diluted 1:500).

Techniques: Transfection, Confocal Microscopy, Staining, Marker

Journal: Scientific Reports

Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways

doi: 10.1038/s41598-018-36374-4

Figure Lengend Snippet: Immune isolation of Tau by mean of the GFP 1–10 sensors. ( a ) Cell lysates from C17.2 cells transfected as indicated are analysed by western blot before (cell lysates) or after immune isolation on anti-GFP magnetic beads (GFP-Trap isolates). This shows specific isolation of S 11 -Tau when co-transfected with GFP 1–10 , but not in the negative controls, including post-lysis mixing of lysates obtained from cells transfected with either S 11 -Tau or GFP 1–10 (lanes labelled with “mix”). β-actin is the loading control for the cell lysates, whereas the presence of the sensor is verified with an anti-GFP antibody. ( b ) Cell lysates and GFP-immune isolates obtained from C17.2 cells transiently transfected with S 11 -Tau and the indicated sensors are analysed by western blot as described for ( a ). Molecular weight markers are given on the left of the blots, full blots are shown scanned by dual infrared imaging.

Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), pATM (Cell Signaling, S1981, diluted 1:500), pChk1 (Cell Signaling, S345, diluted 1:500), pChk2 (Cell Signaling, T68, diluted 1:500), pATR (Cell Signaling, S428, diluted 1:500).

Techniques: Isolation, Transfection, Western Blot, Magnetic Beads, Lysis, Control, Molecular Weight, Imaging

Journal: Nutrients

Article Title: Influence of N-Acetylcysteine Supplementation on Physical Performance and Laboratory Biomarkers in Adult Males: A Systematic Review of Controlled Trials

doi: 10.3390/nu15112463

Figure Lengend Snippet: Studies included in the systematic review of the effect of N-Acetylcysteine supplementation on health biomarkers.

Article Snippet: Rhodes et al, 2019, Australia [ ] , Double-blind, pre-post, placebo-controlled , 17 ♂ Semi-professional/Semi-elite rugby players Age (mean ± SD) 20.4 ± 0.9 years Weight (mean ± SD) 103.0 ± 12.0 kg Height (mean ± SD) 182.3 ± 7.4 cm Yo-Yo Intermittent Recovery Test Level 1 (mean ± SD) 17.4 ± 1.73 Level Study withdrawals: 4 6 participants IG 7 participants CG , IG: 1 g/day (2 × 50 mg capsules) NAC (Nutrabio Labs Inc., Middlesex, NJ, USA) CG: 1 g (2 × 50 mg capsules) of placebo (sucrose and salt mixture) For 6 days. , Muscle soreness Broken bronco shuttle test Fastest shuttle time (High-intensity exercise) Side effects , ↑ Muscle Soreness ↔ Broken bronco shuttle test ↑ Fastest shuttle time ↔ Side Effects.

Techniques: Activity Assay, Nitration